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In-vitro and In-vivo Studies (2008, July) !
Although it was first thought that it was only the acidic nature of this herb that preventsbacteria from flourishing, it is now established that Hibiscus sabdariffa contains compounds
that prevent the adhesion of E. coli to the bladder wall lining: the calyx contains substantial
quantities of polyphenols: flavonoids, sambubioanthocyanidins and proanthocyanidins, reported by several studies to possess excellent antimicrobial activity against organisms like Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Pseudomonas aeruginosa with similar activity to chloramphenicol. This activity has been revealed to be due to polyphenolic nature of the flavonoïds as well as other ingredients: polysaccharides and a high concentration of simple organic acids.
After the ex-vivo and in-vitro studies proving
a very high efficiency (also compared to other existing products), the
in-vivo study is running for a new claim. Also unofficial trials proved
the curative properties (and not only preventive).
Evaluation of the antimicrobial preservation efficiency : Utirose shows great effects on the reduction of an initial microbial contamination either for E. coli [from 2,8 107 to 0], and C. albicans [from 1,6 107 to 103]. Then, this antimicrobial effect occurs immediately, and permits to obtain a total decontamination of the medium in 1 day after the beginning of the treatment (Protocol of the USP XXV Pharmacopeia).
Determination of Anti-microbial activity in solid medium (adaptation from Centers for Disease Control and Prevention / US Department of Health & Human Services / USA.gov) : Utirose gives very significant result on the antimicrobial efficiency on E. coli and C. albicans on solid medium. Comparison with standard commercial cranberry extract shows that Utirose is better in antimicrobial activity (and much better against Candia Albicans).
According to the Globally Harmonised System (GHS), UTIROSE is classified in the hazard Category 5 (or unclassified, which means non-toxic) : Acute toxic class method [OECD Guidelines 423 (17/1 Z/2001)]
After a single ingestion, Utirose shows a low bioavailability, which means that molecular compounds are eliminated by urinary excretion. New polyphenol compounds appear in urine of rat after single ingestion of Utirose. Some compounds come directly from Utirose, some compounds come from metabolism induced by ingestion of Utirose, and some compounds are new products coming from metabolism of Utirose molecules, for example by methylation, sulfonation or glycosylation. Utirose compounds are available in urinary tract where its antimicrobial activity is efficient. We also checked the urine acidification : pH of 5.5-6 after ingestion versus 7.5-8.0 without.
In-vivo study confirms the Urinary comfort improvement (Prevention of urinary tract infections, Limitation of risks of infections with repetition) and so to get the official claim. End results with subjectives data have been finalized on September 2008. The protocol of this in-vivo study concerns 90 women including 30 with placebo - and the other with 200mg of UTIrose. The objective results (diminution of infections) show a diminution by 77% with a very significant p-value (p<0.001).
Nota : Aqueous preparations of Hibiscus sabdariffa calyces are acidic, antibacterial beverages widely used in folk medicine in Mali to acidify and deodorize the urine and to protect against urinary infections as well as kidney disorders (formation of stones).
Evaluation of the antimicrobial preservation efficiency : Utirose shows great effects on the reduction of an initial microbial contamination either for E. coli [from 2,8 107 to 0], and C. albicans [from 1,6 107 to 103]. Then, this antimicrobial effect occurs immediately, and permits to obtain a total decontamination of the medium in 1 day after the beginning of the treatment (Protocol of the USP XXV Pharmacopeia).
Determination of Anti-microbial activity in solid medium (adaptation from Centers for Disease Control and Prevention / US Department of Health & Human Services / USA.gov) : Utirose gives very significant result on the antimicrobial efficiency on E. coli and C. albicans on solid medium. Comparison with standard commercial cranberry extract shows that Utirose is better in antimicrobial activity (and much better against Candia Albicans).
According to the Globally Harmonised System (GHS), UTIROSE is classified in the hazard Category 5 (or unclassified, which means non-toxic) : Acute toxic class method [OECD Guidelines 423 (17/1 Z/2001)]
After a single ingestion, Utirose shows a low bioavailability, which means that molecular compounds are eliminated by urinary excretion. New polyphenol compounds appear in urine of rat after single ingestion of Utirose. Some compounds come directly from Utirose, some compounds come from metabolism induced by ingestion of Utirose, and some compounds are new products coming from metabolism of Utirose molecules, for example by methylation, sulfonation or glycosylation. Utirose compounds are available in urinary tract where its antimicrobial activity is efficient. We also checked the urine acidification : pH of 5.5-6 after ingestion versus 7.5-8.0 without.
In-vivo study confirms the Urinary comfort improvement (Prevention of urinary tract infections, Limitation of risks of infections with repetition) and so to get the official claim. End results with subjectives data have been finalized on September 2008. The protocol of this in-vivo study concerns 90 women including 30 with placebo - and the other with 200mg of UTIrose. The objective results (diminution of infections) show a diminution by 77% with a very significant p-value (p<0.001).
Nota : Aqueous preparations of Hibiscus sabdariffa calyces are acidic, antibacterial beverages widely used in folk medicine in Mali to acidify and deodorize the urine and to protect against urinary infections as well as kidney disorders (formation of stones).